Plasmid Isolation Kit from Bacterial cells (Teaching) 25expt SRL
27725
Part E
Storage : Includes components ranging from RT to -20°C
Shelf Life : 6 Months
HSN Code : 38229090
IMDG Identification :Not Regulated for Transport (Non-Haz)
Storage : Includes components ranging from RT to -20°C
Shelf Life : 6 Months
HSN Code : 38229090
IMDG Identification :Not Regulated for Transport (Non-Haz)
Description:
The overall goal is to isolate extra-chromosomal, plasmid DNA from bacterial cells that contain the plasmid. The cells are first re-suspended in a suitable buffer (that minimizes nuclease activity) and ruptured to release the cellular contents. Subsequently, the plasmid DNA and RNA are separated from genomic DNA. Finally, by decreasing the “effective concentration” of water, the plasmid DNA is precipitated through sheer desolvation effects. If desired, the contaminating RNA can be removed by digestion with RNase A.
The overall goal is to isolate extra-chromosomal, plasmid DNA from bacterial cells that contain the plasmid. The cells are first re-suspended in a suitable buffer (that minimizes nuclease activity) and ruptured to release the cellular contents. Subsequently, the plasmid DNA and RNA are separated from genomic DNA. Finally, by decreasing the “effective concentration” of water, the plasmid DNA is precipitated through sheer desolvation effects. If desired, the contaminating RNA can be removed by digestion with RNase A.
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