SPINeasy® DNA Pro Kit for Feces 50preps MP
Key features and details
Soil and fecal samples display distinct processing challenges. The composition of fecal samples is largely dependent on diet which includes fibers, undigested particle, bilirubin, complex polysaccharides, and lipids.
Fecal samples display distinct processing challenges. The composition of fecal samples is largely dependent on diet which includes fibers, undigested particles, bilirubin, complex polysaccharides, and lipids. Those compounds impair the sample homogenization, decrease both the quantity and quality of the DNA extracted.
The SPINeasy® DNA Pro Kit for Feces was designed to tackle these challenges by providing an efficient way for rapidly isolating high-quality DNA from feces samples. Irrespective of the complexities, samples are optimally homogenized by bead beating with the new Lysing Matrix YB and lysis Buffer SF1. Subsequent treatment with Buffer SF2 effectively removes humic acid and other contaminants. The chemistry included in Buffer SF3 enables the specific binding of DNA without co-purification of RNA, eliminating the need for RNase A treatment. DNA obtained from fecal samples showed no inhibition in PCR and was immediately ready-to-be use for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome) without the need for a further inhibitor removal step.
Application Notes
The SPINeasy® DNA Pro Kit for Feces was designed for rapidly isolating high-quality DNA from feces samples.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Recommended
- Stool Sample
Product Details
| SKU | 116547050 |
| Application Notes | The SPINeasy® DNA Pro Kit for Feces was designed for rapidly isolating high-quality DNA from feces samples. |
| Base Catalog Number | 6547050 |
| Components | Lysing Matrix YB (50 ea), Equilibration Buffer (12 mL), Buffer SF1 (54 mL), Buffer SF2 (12 mL), Buffer SF3 (48 mL), Buffer SF4 (40 mL), Buffer SF5 (40 mL), Buffer SF6 (7.5 mL), Column SF (50 ea), Collection Tube (50 ea x 3), Elution Tube (50 ea), 2 mL Tube (100 ea) |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Storage | Store at Room Temperature (15-25°C), Dry environment. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
