SPINeasy® Plasmid Midiprep Kit 25preps MP
Key features and details
Quick and efficient purification of both high-copy and low-copy number plasmid DNA from 25-50mL of transformed bacterial culture using silica-membrane spin-colum.SPINeasy Plasmid Midiprep Kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 – 50 mL of transformed bacterial culture. Using our specially formulated buffers, bacterial cells are disrupted by alkaline lysis to release plasmid DNA. Cell debris, such as chromosomal DNA and proteins, are precipitated by neutralization and cleared through centrifugation. Subsequently, plasmid DNA in the lysate supernatant is adsorbed on a silica membrane, washed and eluted in a convenient process using the spin column technology. Up to 1 mg of plasmid DNA can be purified through this simple workflow, which is immediately ready for routine molecular biology laboratory applications.
Application Notes
The SPINeasy Plasmid Midiprep Kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 – 50 mL of transformed bacterial culture.
Usage Statement
Research Use Only (RUO).
| SKU | 116539025 |
| Application Notes | The SPINeasy Plasmid Midiprep Kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 – 50 mL of transformed bacterial culture. |
| Base Catalog Number | 65390 |
| Components | 60ml Resuspension Buffer SD1, 60ml Alkaline Lysis Buffer SD2, 90ml Neutralization Buffer SD3, 70ml Wash Buffer DW1, 12ml Wash Buffer DW2, 30ml Elution Buffer SD, 600µl RNase A, 25x Column SD with collection tubes |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 25 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Storage | Store at Room Temperature (15-30℃), Dry environment |
| Usage Statement | Research Use Only (RUO). |