Premix Ex Taq™ (Probe qPCR) 200rxn TaKaRa
Non-returnable
₹14,350.00
RR390A
Contains
1. Premix Ex Taq (Probe qPCR) 2X- 1mlx5
2. ROX Reference Dye 50X 200μl
3. ROX Reference Dye II 50X 200μl
2. ROX Reference Dye 50X 200μl
3. ROX Reference Dye II 50X 200μl
Rapid and accurate PCR product detection
Quantitative gene expression analysis using real-time PCR (qPCR)
2X master mix enables easy pipetting
Optimized buffer increases amplification efficiency, highly sensitive probe-based qPCR assays, and 5' nuclease assays
Included Tli RNase H minimizes PCR inhibition by removing residual mRNA in input cDNA
Applications
Real-time PCR (qPCR) quantification of DNA using methods such as probe-based qPCR assays and 5' nuclease assays
Premix Ex Taq (Probe qPCR) is a 2X premix for real-time PCR (qPCR) detection with probe-based qPCR or 5' nuclease assays. The 2X premix includes Takara Ex Taq HS, which contains a hot start PCR enzyme with an anti-Taq antibody, and a buffer optimized for real-time PCR. The resulting premix allows excellent suppression of non-specific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. In addition, Tli RNaseH, a heatresistant RNaseH, is included to minimize PCR inhibition from residual mRNA in reactions using cDNA templates. This product is excellent for high-speed PCR and allows accurate target quantification and detection over a broad dynamic range, making it possible to conduct highly reproducible and reliable real-time PCR analyses.