PrimeScript™ 1st strand cDNA Synthesis Kit 50rxn TaKaRa
Non-returnable
₹12,400.00
6110A
Contains
1. PrimeScript RTase 200U/μl 50μl
2. 5X PrimeScript Buffer 200μl
3. RNase Inhibitor 40U/μl 25μl
4. dNTP Mixture 10mM each 50μl
5. Oligo dT Primer 50μM 50μl
6. Random 6 mers 50μM 100μl
7. RNase Free dH2O 1ml
2. 5X PrimeScript Buffer 200μl
3. RNase Inhibitor 40U/μl 25μl
4. dNTP Mixture 10mM each 50μl
5. Oligo dT Primer 50μM 50μl
6. Random 6 mers 50μM 100μl
7. RNase Free dH2O 1ml
- This cDNA synthesis kit includes oligo dT and random 6-mer primers
- Strong strand displacement and extension capability: PrimeScript RT is capable of synthesizing long and full-length cDNA molecules (up to 12 kb)
- High specificity: synthesize cDNA at 42°C—a temperature that does not promote RNA degradation—resulting in low background and higher yields
- Outstanding accuracy: lowest error rate among five commercially available reverse transcriptases tested
- Easy to use: low rates of non-specific annealing, even on incompletely denatured RNA
- Works on challenging templates: excellent results even with templates that are GC-rich or have high levels of secondary structure
- Highly sensitive: use less of your precious RNA samples
- Demonstrated success with RT reaction times of 30 min and 60 min (for longer transcripts)
The PrimeScript 1st strand cDNA Synthesis Kit contains all the reagents necessary to synthesize first strand cDNA from total or polyA+ RNA using PrimeScript RTase, a MMLV (Moloney Murine Leukemia Virus)-derived reverse transcriptase. This enzyme is capable of synthesizing cDNA up to 12 kb in length. PrimeScript RTase can synthesize cDNA efficiently at 42℃, even from RNA templates that contain GC-rich regions or complex secondary structures, making it unnecessary to perform reactions at high temperatures that may degrade RNA. The first strand cDNA synthesized with this kit can be used for variety of applications, including second strand synthesis, hybridization, PCR amplification, and real-time PCR.