PrimeScript™ RT Reagent Kit (Perfect Real Time) 200rxn TaKaRa
2. PrimeScript RT Enzyme Mix I 100μl
3. Oligo dT Primer 50μM 100μl
4. Random 6 mers 100μM 400μl
5. RNase Free dH2O 1ml
6. EASY Dilution (for Real Time PCR) 1ml
Allows fast, efficient synthesis of cDNA for real-time PCR. This kit is designed for two-step real-time RT-PCR.
Includes random 6-mers and oligo dT primer for use as reverse transcription primers. The reaction can be performed using one or a mixture of these two primer types; alternately, gene-specific primers may be used.
Includes EASY Dilution (for Real Time PCR) when generating reference standards for a qPCR standard curve. This reagent facilitates higher accuracy at lower standard concentrations.
The PrimeScript RT Reagent Kit (Perfect Real Time) includes PrimeScript reverse transcriptase for strong strand-displacement activity and efficient synthesis of cDNA for real-time PCR. PrimeScript RTase is a modified, recombinant MMLV (Moloney murine leukemia virus) reverse transcriptase and is verified to be RNase H-minus.
PrimeScript RTase is also robust, versatile, and can be used for reverse transcription of virtually any RNA template, including templates that have a high degree of secondary structure or are GC rich. Because of the excellent extension capability of PrimeScript, cDNA synthesis reactions can be performed at 42°C, minimizing the risk of RNA degradation that often occurs at higher temperatures.buffer