NucleoSpin RNA Plant and Fungi (50) MACHEREY-NAGEL
- Universal kit for challenging plant and fungal samples
- Tailored protocols for diverse starting materials
- Filter columns for lysate clearing included
| Application | Isolation of RNA |
| Selling unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Target | RNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Mini prep |
| Handling | Centrifugation, vacuum |
| Lysate clarification | Centrifugation, NucleoSpin Filter |
| Automated use | No |
| Sample material | Fungi, Plant |
| Sample amount | < 500 mg |
| Fragment size | > 200 nt |
| Typical yield | 20–70 µg |
| Theoretical binding capacity | 200 µg |
| Typical purity A260/A280 | 1.9–2.1 |
| Typical purity A260/A230 | Approx. 2 |
| Typical RIN | 7−9 |
| Elution volume | 50 µL |
| Preparation time | 25 min/6 preps |
| Typical downstream application | Gene expression profiling, Northern blotting, Primer extension, Real−time RT−PCR |
| Storage temperature | 15–25 °C / 59–77 °F |
| Shelf life (from production) | 24 Month(s) |
The following table shows a selection of samples that were successfully tested with NucleoSpin® RNA Plant and Fungi:
Species | Material |
Thale cress (Arabidopsis thaliana) | Leaves, seeds, siliques, roots |
Alpine rock-cress (Arabis alpina) | Leaves, stems |
Tobacco (Nicotiniana tabacum) | Leaves |
Wheat (Triticum aestivum) | Leaves |
Barley (Hordeum vulgare) | Roots |
Maize (Zea mays) | Grains, leaves |
Fir (Abies procera) | Needles |
Spruce (Picea abies) | Needles |
Populus (Populus sp.) | Leaves, stems, roots |
Potato (Solanum tuberosum) | Tuber |
Grapevine (Vitis vinifera) | Leaves, grapes |
Sugar beet (Beta vulgaris) | Roots |
Sugar cane (Saccharum officinarum) | Stalks |
Pea (Pisum sativum) | Roots |
Alfalfa (Medicago sativa) | Roots |
Clover (Medicago truncatula) | Roots, leaves |
Banana (Musa sp.) | Pulp |
Kiwi (Actinidia deliciosa) | Pulp |
Citrus fruits (Citrus sp.) | Pulp |
Blueberry (Vaccinium myrtillis) | Pulp |
Apple (Malus domestica) | Pulp |
Ginger (Zingiber officinale) | Rhizome |
Mushroom (Laccaria bicolor) | Mycelium |
Mushroom (Agaricus campestris) | Fruiting body |
Grey mold (Botrytis cinerea) | Mycelium |