SYBR Green qPCR Master Mix, 200 x 25 μL rxns/ 500 x 10μL rxns GENEI
Description
SYBR Green qPCR Master Mix is an optimized ready-to-use solution for qPCR. The master mixes include Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The use of SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. SYBR Green qPCR Master Mixes are compatible with most real-time thermalcyclers
Applications
• Gene expression, siRNA validation, Genotyping, Pathogen detection
Specifications:
- Sensitivity—detects low copy number targets
- Specificity— Hot Start TaqDNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Wide linear range—accurate quantification across 9 orders of magnitude
- Reproducibility and convenience—ready-to-use 2x master mix
SYBR Green/ROX qPCR Master Mix (2x) includes Hot Start Taq DNA Polymerase and dNTPs(also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water
Number of Reactions: 200 reactions
Polymerase: Taq DNA Polymerase
Detection Method: SYBR
PCR Method: qPCR
Sample Type (General): DNA, dsDNA
- 2 x 1.25 mL SYBR Green/ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 µL reactions.
- 2 x 1.25 mL Nuclease-Free Water
Store at -20°C.