Taq DNA Polymerase (5 U/μl) (Includes Enzyme: 1 vial; 10X Taq Buffer B: 4 vials; 25 mM MgCl2: 4 vials), 1000Units GeNei

Description

Taq DNA Polymerase is a 94 kD thermostable enzyme. Its optimum temperature of activity is between 55° C and 75° C. The enzyme lacks 3′ to 5′ exonuclease (proof reading) activity but has an inherent 5′ to 3′ exonuclease activity.

Specification :
The enzyme is supplied at concentrations of 1 U/μl, 3 U/μl, 5 U/μl.
Taq DNA Polymerase Buffers : Refer the chart for buffer composition.
Buffer supplied at 10X concentration.

Storage and Dilution buffer :
Tris-HCl (pH 8), KCl, EDTA, DTT, Tween 20, Igepal and Glycerol.

Storage : -20°C

Application :
• Amplification of DNA fragments of varied sizes ranging from about 100 bp to 3kb by polymerase chain reaction.
• The enzyme can be used in RAPD studies to detect polymorphism in several species.
• Taq DNA Polymerase can be supplied with :
• 10X buffer containing 15 mM MgCl₂ or
• 10X buffer and separate vial of 25 mM MgCl₂

Note : Concentration of the components of the buffer are proprietary.

Performance Test :
1. Taq DNA Polymerase is extensively tested for its performance in several PCR Amplifications, RAPD, RT-PCR.
2. Taq DNA Polymerase is also tested in nested PCR using WSSV detection kit

Storage : -20°C