PrimeScript™ RT-PCR Kit 50rxn TaKaRa
Non-returnable
₹20,500.00
RR014A
Contains
1. PrimeScript RTase (for 2 step) 25μl
2. 5X PrimeScript Buffer 200μl
3. RNase Inhibitor 40U/μl 25μl
4. dNTP Mixture 10 mM each 150μl
5. Oligo dT Primer 2.5 μM 50μl
6. Random 6 mers 20 μM 50μl
7. TaKaRa Ex Taq® HS 5U/μl 25μl
8. 10X PCR Buffer II 250μl
9. Control F-1 Primer 20μM 10μl
10. Control R-1 Primer 20μM 10μl
11. Positive Control RNA 2x10^5 copies/μl 20μl
12. RNase Free dH2O 1ml
2. 5X PrimeScript Buffer 200μl
3. RNase Inhibitor 40U/μl 25μl
4. dNTP Mixture 10 mM each 150μl
5. Oligo dT Primer 2.5 μM 50μl
6. Random 6 mers 20 μM 50μl
7. TaKaRa Ex Taq® HS 5U/μl 25μl
8. 10X PCR Buffer II 250μl
9. Control F-1 Primer 20μM 10μl
10. Control R-1 Primer 20μM 10μl
11. Positive Control RNA 2x10^5 copies/μl 20μl
12. RNase Free dH2O 1ml
- RT-PCR kit includes all reagents needed for two-step endpoint RT-PCR (e.g., where products are analyzed using gel electrophoresis)
- Robust reverse transcription and amplification even from challenging RNA templates
- Complete elongation of complex RNA templates
- High-yield transcription of full-length cDNA up to 12 kb in length
- Minimal false priming and robust amplification due to the combination of PrimeScript RT with Takara Ex Taq HS
The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RT, an RNase H Minus MMLV reverse transcriptase enzyme that provides robust reverse transcription of virtually any RNA template. This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RT, and Takara Ex Taq HS polymerase. PrimeScript RT enables full-length cDNA synthesis from GC-rich and even highly structured RNA templates, while Takara Ex Taq HS allows for efficient, sensitive, and specific cDNA amplification. Reverse transcription can be performed at 42°C, avoiding the risk of RNA degradation, which may occur at higher temperatures. The PrimeScript RT-PCR Kit generates high-yield, full-length cDNA up to 12 kb in length.